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assayed in a uniport olfactometer [12] (ANOVA followed by Tukey's. HSD, all can be downloaded as Additional file 2. Mapping statistics for each library can be found in Additional file. 4, and TPM values for each replicate library can be.
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No HIGH-Affinity K+ Uptake (HAK) genes showed differential up-regulation in sos1 roots compared with that in the wild type according to microarray data. Then, you take the best hit and fetch the uniprot id (id mapping tools from uniprot). To match coordinates of your protein with the one linked with the uniprot id the best is to perform an global pairwise alignment (see needle).